Additional file 2.
Figure S2. E2 induced upregulation of bcl-2, cyclin D1 and survivin at both transcriptional and the post-transcriptional level. (A) MCF-7 cells were pretreated or not with 2 μg/ml actinomycin D for 1 h and then stimulated with 10nM E2 for 12 h. Total protein was extracted to determine the expression of bcl-2, cyclin D1 and survivin. (B) The densitometry of each gene vs. β-actin was indicated and statistical analysis was shown. * denotes P < 0.05 compared with control (the first group).
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Yu et al. BMC Cancer 2012 12:29 doi:10.1186/1471-2407-12-29