E2 reduced miR-16, miR-143 and miR-203 expression in MCF-7 cells. MCF-7 cells were treated as described in Figure 1 and stimulated by E2 for 3, 6, 24 and 48 h. Total RNA was extracted and miRNA expression levels were examined by QPCR. MiR-631 is a non-E2 responsive miRNA serving as negative control. * denotes P < 0.05 compared with vehicle control.
Yu et al. BMC Cancer 2012 12:29 doi:10.1186/1471-2407-12-29