Figure 2 .
EGCG blocks phosphorilation of EFGR, HER2, ERK1/2, AKT and mTOR in A549 cells. A549 cells were treated for 6, 12, 24 and 48 hours with C75 (72 μM) and 6, 12 and 24 hours with EGCG (265 μM), and equal amounts of lysates were immunoblotted with anti-EGFR, anti-HER2, anti-ERK1/2, anti-AKT, anti-mTOR and anti-FASN antibodies. Activation of the protein under study was analyzed by assessing the phosphorylation status using the corresponding phospho-specific antibody. Total amounts of HER2 and AKT proteins remain unchanged. Blots were reproved with an antibody for β-actin to control for protein loading and transfer. Gels shown are representative of those obtained from three independent experiments.
Relat et al. BMC Cancer 2012 12:280 doi:10.1186/1471-2407-12-280