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Gene expression in tumor cells and stroma in dsRed 4T1 tumors in eGFP-expressing mice with and without enhanced oxygenation

Ingrid Moen1*†, Charlotte Jevne1†, Jian Wang1, Karl-Henning Kalland2,3, Martha Chekenya1, Lars A Akslen2,4, Linda Sleire1, Per Ø Enger1, Rolf K Reed1, Anne M Øyan2,3 and Linda EB Stuhr1

1 Department of Biomedicine, University of Bergen, Jonas Lies vei 91, 5009 Bergen, Norway

2 The Gade Institute, University of Bergen, Bergen, Norway

3 Department of Microbiology, Haukeland University Hospital, Bergen, Norway

4 Department of Pathology, Haukeland University Hospital, Bergen, Norway

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BMC Cancer 2012, 12:21 doi:10.1186/1471-2407-12-21

Published: 17 January 2012

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Additional file 1:

Figure S1 Significantly changed genes in the tumor and stromal compartment. The number of significantly expressed genes (FDR < 5%) in tumor cells and stromal cells treated with daily and intermittent hyperbaric oxygen (HBO), both compared to control cells. Overlapping genes in the two different compartments are shown in red.

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Additional file 2:

Table S1. Cellular processes, pathways and molecular function. Gene set enrichment analysis (GSEA) after intermittent hyperbaric oxygen (HBO) treatment of tumor cells.

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Additional file 3:

Table S2. Cellular processes, pathways and molecular function. Gene set enrichment analysis (GSEA) after daily hyperbaric oxygen (HBO) treatment of tumor cells.

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Additional file 4:

Table S3. Cellular processes, pathways and molecular function. Gene set enrichment analysis (GSEA) after intermittent hyperbaric oxygen (HBO) treatment of stroma cells.

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Additional file 5:

Table S4. Cellular processes, pathways and molecular function. Gene set enrichment analysis (GSEA) after daily hyperbaric oxygen (HBO) treatment of stroma cells.

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Additional file 6:

Figure S2 Tumor growth and metastasis in long-term HBO treated tumors. 4T1 mammary tumor growth (% of initial volume) over 14 days in long-term intermittent (n = 7) and long-term daily (n = 7) hyperbaric oxygen (HBO) treated tumors. Intermittent treatments were given at day 1, 4 and 7, 10 and 13. Data represent mean ± SEM. B) Histological section displaying lung metastasis after (B) long-term intermittent HBO and (C) long-term daily HBO treatment (both × 100).

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