Hypoxia extended G0/G1 status with relatively slow growth of cells. Both OVCAR-3 and ES-2 cells were maintained under either hypoxia or normoxia for variable periods of times. (A) Growth curves show that the cells under hypoxia grew relatively slower than those cells under normoxia (P < 0.01). (B) Cell cycle analyses show extended G0/G1 phase in the cells cultivated under hypoxia, and the corresponding histogram shows statistically significantly increased G0/G1 stage in both cell lines (P < 0.01 for OVCAR-3 and P < 0.05 for ES-2).
Liang et al. BMC Cancer 2012 12:201 doi:10.1186/1471-2407-12-201