Western blot showing the level of vascular endothelial growth factor (VEGF) in MDA-MB-231 cells was dramatically reduced following the treatment of MSeA at 5 μM for 12 hrs. A higher MSeA concentration (10 μM) or longer treatment period (24 hrs) did not further inhibit the VEGF production. Commercial antihuman VEGF antibody (1: 200XD) was used to visualize the cellular VEGF protein band at the given MSeA concentrations and treatment time periods.
Wu et al. BMC Cancer 2012 12:192 doi:10.1186/1471-2407-12-192