Table 1

Quantitative detection of CTCs in tumour bearing animals
 Model Tumour size CTC
(Application) (Animals) (cells/ml)
MDA-MB-231 20mm2 0/5
(orthotopic) (5)
MDA-MB-231 50 mm2 0/5
(orthotopic) (5)
MDA-MB-231 80 mm2 3/5
(orthotopic) (5) (5,10,and 15)
MDA-MB-231 120 mm2 1/5
(orthotopic) (5) (7)
MDA-MB-231 20-120 mm2 0/20
(subcutaneous) (20)
 KPL-4 50 mm2 0/3
(orthotopic) (3)
 KPL-4 80 mm2 1/2
(orthotopic) (2) (20)
 KPL-4 120 mm2 1/4
(orthotopic) (4) (15)

CTCs could be quantified by qRT-PCR targeting human Alu-sequences in 6 out of 29 blood samples gained from xenografted animals. CTCs were detectable at tumour sizes between 80 to 140 mm2 (MDA-MB-231 and KPL-4). No CTCs could be detected in a subcutaneous MDA-MB-231 model. Number of CTCs were calculated by spiking rows which were included in every run.

Gorges et al.

Gorges et al. BMC Cancer 2012 12:178   doi:10.1186/1471-2407-12-178

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