Tet-On system-controlled sTRAIL expression induces tumor cell apoptosis in vivo. The xenograft tumor cells were isolated from the animals administered with rAAV-TRE-TRAIL&rAAV-Tet-On and lyzed with lysis buffer. 400 micrograms of total protein in the lysed were subjected to SDS-PAGE. Cleavage of procaspase-8 and procaspase-3 were examined by Western blot assay using corresponding specific antibodies. β-actin was used as loading control. The tumor lysates from the animals administrated with rAAV-TRE-EGFP&rAAV-Tet-On and rAAV-CAG-TRAIL were used as negative and positive control, respectively.
Zheng et al. BMC Cancer 2012 12:153 doi:10.1186/1471-2407-12-153