Additional file 2.
Figure S2. Quantification of the induction of apoptosis upon exposure of Capan-2 spheroid to Gemcitabine and CHIR-124. Analysis was performed as described in Figure 6. Apoptosis was revealed by immunodetection of cleaved form of PARP. Spheroid sections were analyzed to quantify the number of cells with cleaved PARP using the FIJI software (measure plugin). The data are expressed as the percentage of spheroid sections displaying 0 to 10, 10 to 20, or more than 20 PARP-C positive cells.
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Dufau et al. BMC Cancer 2012 12:15 doi:10.1186/1471-2407-12-15