Gemcitabine and CHIR-124 produce a synergistic cytotoxic effect on Capan-2 spheroid. Spheroids were treated for 72 h after 4 day initial culture with gemcitabine, CHIR-124 or a combination of the 2 compounds at concentration corresponding to their respective EC20 values. Spheroid viability was quantified at day 7 by ATP monitoring with the luminescent ATPlite assay. ATP content percentage was calculated with regard to non-treated spheroid and showed cell growth inhibition and/or toxicity.
Dufau et al. BMC Cancer 2012 12:15 doi:10.1186/1471-2407-12-15