Table 1

TNBC-TICs exhibit a greater clonogenic growth potential as compared to TNBC, TPBC-TICs, TPBC or parental 4T1 cells

Cell type1

Number of cells transplanted

Fraction of mice with tumors2


TPBC-derived:

CD24+/ALDH1+/CD44high

500

5/5

CD24+/ALDH1+/CD44high

100

5/5

CD24+/ALDH1+/CD44high

50

4/5

CD24+/ALDH1+/CD44high

25

2/5

CD24+/ALDH1+/CD44high

10

3/5

TPBC-derived:

CD24+/ALDH1-/CD44low

500

1/5

CD24+/ALDH1-/CD44low

100

0/5

CD24+/ALDH1-/CD44low

50

0/5

CD24+/ALDH1-/CD44low

25

0/5

CD24+/ALDH1-/CD44low

10

0/5

TNBC-derived:

CD24+/ALDH1+/CD44high

500

5/5

CD24+/ALDH1+/CD44high

100

5/5

CD24+/ALDH1+/CD44high

50

5/5

CD24+/ALDH1+/CD44high

25

5/5

CD24+/ALDH1+/CD44high

10

4/5

TNBC-derived:

CD24+/ALDH1-/CD44low

500

1/5

CD24+/ALDH1-/CD44low

100

0/5

CD24+/ALDH1-/CD44low

50

0/5

CD24+/ALDH1-/CD44low

25

0/5

CD24+/ALDH1-/CD44low

10

0/5


1Tumor cells derived from TPBC or TNBC cells were stably transfected with eGFP using the lentivirus transduction technique as described in detail in the Methods section

2Twenty-one days post tumor cell transplantation into the mammary pad of naïve female BALB/c mice, animals were scarified and eGFP signal from the tumors were measured using the Maestro™ in vivo imaging system (CRI), and spectral unmixing was performed to segregate skin and hair auto fluorescence and to measure the true eGFP signal, as described in detail in the Methods section. Data is the fraction of mice with tumors (n = 5)

Kaur et al. BMC Cancer 2012 12:120   doi:10.1186/1471-2407-12-120

Open Data