Open Access Research article

Why do results conflict regarding the prognostic value of the methylation status in colon cancers? the role of the preservation method

Benjamin Tournier12*, Caroline Chapusot123, Emilie Courcet12, Laurent Martin24, Côme Lepage13, Jean Faivre13 and Françoise Piard1234

Author Affiliations

1 Institut National de la Sante et de la Recherche Médicale, Université de Bourgogne, U866 Dijon, France

2 Service de Pathologie, CHU, Dijon, France

3 Registre Bourguignon des Cancers Digestifs, CHU, Dijon, France

4 Centre de ressources biologiques Ferdinand Cabanne, Dijon, France

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BMC Cancer 2012, 12:12  doi:10.1186/1471-2407-12-12

Published: 13 January 2012

Additional files

Additional file 1:

This file is in Microsoft Word 97-2003 format. It includes a detailed description of the protocol for the extraction method dedicated to FFPE tissue and of the protocol for the DNA bisulfite conversion

Format: DOC Size: 26KB Download file

This file can be viewed with: Microsoft Word Viewer

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Additional file 2:

This file is in Microsoft Word 97-2003 format. This file contains the LINE-1 primer sequences (Supplementary table 2), the dispensation orders of nucleotides for the three markers (Supplementary table 2) and data on the LINE-1 methylation levels obtained from FFPE DNA extracted by the classical method (Supplementary table 3).

Format: DOC Size: 70KB Download file

This file can be viewed with: Microsoft Word Viewer

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Additional file 3:

This file is in JPEG format. This figure is entitled: Pyrograms of the LINE-1 methylation marker for FFPE DNA n°2 extracted with the classical method. It groups the two pyrograms obtained from the two LINE-1 analyses of the FFPE DNA n°2 extracted with the classical method.

Format: JPEG Size: 177KB Download file

Open Data