Expression of miR-135a was elevated in human breast tumors with metastasis. (A) miR-135a levels were detected in 30 human breast samples by SYBR Green qRT-PCR with 18S rRNA used as a loading control. For benign tumors (adenosis or fibroadenoma) n = 10 and for „triple negative" invasive malignant tumors (ER (-), PR (-) and CerbB-2 (-) markers are all negative and all have lymph node metastasis positive) n = 20. p-values were obtained using a 2-sided Student"s t-test. (B) Endogenous miR-135a detected in breast cancer cell lines by poly-A RT-PCR. U6 was used as the control.
Chen et al. BMC Cancer 2012 12:111 doi:10.1186/1471-2407-12-111