Open Access Highly Accessed Research article

Differential expression of THOC1 and ALY mRNP biogenesis/export factors in human cancers

María S Domínguez-Sánchez1, Carmen Sáez2, Miguel A Japón2, Andrés Aguilera1 and Rosa Luna1*

Author Affiliations

1 Centro Andaluz de Biología Molecular y Medicina Regenerativa CABIMER, Universidad de Sevilla-CSIC, Av. Américo Vespucio s/n, 41092 Sevilla, Spain

2 Instituto de Biomedicina de Sevilla (IBIS) and Department of Pathology, Hospital Universitario Virgen del Rocío/Universidad de Sevilla/CSIC, Av. Manuel Siurot s/n, 41013 Sevilla, Spain

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BMC Cancer 2011, 11:77  doi:10.1186/1471-2407-11-77

Published: 17 February 2011

Additional files

Additional file 1:

Brief description of the pathological diagnoses from the tumor samples included in the tissue array. The table shows a brief description of the pathological diagnoses.

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Additional file 2:

Results of hybridization of the cDNA array. The figure shows the results of hybridization of the cDNA array. THOC1 and ALY are ubiquitously expressed in all normal tissues. The highest THOC1 mRNA levels are observed in thyroid and liver and the lowest in stomach and ovary. ALY probe revealed a similar expression in all normal tissues, with the highest levels in testis.

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Additional file 3:

Expression of THOC1, ALY and SPT4 determined by hybridization of a Cancer Profiling array with THOC1, ALY and SPT4 probes. In the table, it is shown the average of intensity (LAU/mm2) found for the THOC1, ALY or SPT4 hybridization probes in normal tissues (N) or tumor tissues (T) and its corresponding statistical error mean (St. error mean). Results were analyzed by using the two-tailed t-test which compares two paired groups by means of calculating the difference between each set of pairs, and which is based on the assumption that the differences in the entire population follow a Gaussian distribution. Samples marked in grey were found to be statistically significant (p < 0.05).

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Additional file 4:

THOC1 expression in Ovary and Lung tumors. A) Western blot of THOC1 and β actin (as endogenous control) in ovary and lung tissues. B) THOC1 mRNA relative expression in ovary and lung tissues measured by RT-PCR.

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