Image Registration. H&E stained images and IR classified images are first converted into binary images. The IR classified image is overlaid with the H&E stained image by affine transformation, with the optimal matching being found by minimizing the absolute intensity difference between two images. After registration, original annotations (color and/or cell-type information) of each image are restored.
Kwak et al. BMC Cancer 2011 11:62 doi:10.1186/1471-2407-11-62