Effect of PMA treatment on the expression of signaling proteins. All cells were plated and grown overnight in media containing 1% fetal calf serum, except for a control cells that received no treatment (NT) and were grown in media containing 10% fetal calf serum. Cells were then treated with 150 nM PMA for times indicated. Additional control cells were incubated for one hour with either media alone (Media), or with the same concentration of DMSO as was present in the PMA samples (DMSO). Cells were lyzed, and equal amounts of total protein of each sample were subjected to immunoblotting with antibodies against (A) MEK and pMEK; (B) ERK and pERK; (C) c-Src, pSrc(Y416) (pY416) and pSrc(Y527) (Y527); or (E) FAK and pFAK. Western blots of (D) pSrc(Y416) in Chinese hamster ovary (CHO) cells expressing αIIbβ3 and adhered to Fg are also displayed for comparison. One of three representative experiments is shown.
Taherian et al. BMC Cancer 2011 11:293 doi:10.1186/1471-2407-11-293