Cell adhesion to various ECM integrin ligands. Cells (A, MDA-MB-435; B, MDA-MB-231; C, MCF7; and D, Hek-293) were harvested and then untreated (grey box) or 150 nM PMA-treated (black box) cells were allowed to adhere to the plates coated with various ECM proteins for 1 hour. The plates were washed and the number of adhered cells was calculated as a percentage of total added. Mock treated cells, incubated with the same concentration of DMSO as was present in the PMA samples, had similar levels of adhesion as untreated cells (data not shown). One of four representative experiments performed triplicate is shown.
Taherian et al. BMC Cancer 2011 11:293 doi:10.1186/1471-2407-11-293