Figure 1.

(A-D) Quantity and quality of genomic DNA from breast cancer biopsy and PCR amplification of different regions of HPV genome. A: Estimation of quantity and quality of phenol-chloroform extracted genomic DNA from breast cancer biopsies as visualized on an ethidium bromide-stained 1% agarose gel. Lane M: Hind III-digested λ-DNA molecular weight marker. Lane 1-10: Genomic DNA samples from breast cancer biopsies. B: PCR amplification of HPV L-1 consensus sequences showing the amplimer of 450 bp along with β-globin of 268 bp. Lane M: Hae III-digested φ × 174 DNA molecular weight marker. Lane 1: positive control (HPV16 plasmid DNA), Lane 2: HPV16 positive cervical cancer biopsy DNA, Lane 3: Blood DNA from breast cancer patient, Lane 4: HPV negative cell line (C33a) DNA, Lane 5: MCF-7 cell DNA, Lane 6: HeLa cell DNA, Lanes 7-18: breast cancer biopsy DNA. All were positive for β-globin but none of them positive for HPV. C: PCR amplification of HPV16 E6 showing the amplimer of 503 bp along with β-globin of 268 bp. Lane M: Hae III-digested φ × 174 DNA molecular weight marker. Lane 1: positive control (HPV16 plasmid DNA), Lane 2: Cervical cancer DNA, Lane 3: Blood DNA from breast cancer patient, Lane 4: HPV negative cell line (C33a) DNA, Lane 5: MCF-7 cell DNA, Lane 6: Human placental DNA, Lane 7:SiHa cell DNA, Lanes 8-18: breast cancer biopsy DNA showing positivity for β-globin but negative for HPV. D: PCR amplification of HPV16 E7 showing the amplimer of 468 bp along with β-globin of 268 bp. Lane M: Hae III-digested φ × 174 DNA molecular weight marker. Lane 1: positive control (HPV16 plasmid DNA), Lane 2: Cervical cancer DNA, Lane 3: Blood DNA from breast cancer patient, Lane 4: HPV negative cell line (C33a) DNA, Lane 5: MCF-7 cell DNA, Lane 6: Human placental DNA, Lane 7:SiHa cell line DNA, Lane 8-18: breast cancer biopsy DNA showing positivity for β-globin but negative for HPV.

Hedau et al. BMC Cancer 2011 11:27   doi:10.1186/1471-2407-11-27
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