Overview for conducting a Weighted Correlation Network Analyses (WGCNA) of patient TMA data (Steps 1-4) and follow up analyses (Steps 5-7). Steps 1-4 are numbered to correspond with the WGCNA methods section in the text. After defining WGCNA and WGCNA* patient groups, we compare these results to a more conventional variable selection approach (Steps 5-6). Finally, we validate the WGCNA* and conventional results in independent Affymetrix gene expression data sets (Step 7).
Presson et al. BMC Cancer 2011 11:230 doi:10.1186/1471-2407-11-230