Methylation analysis of promoter regions by COBRA. Photographs are representative of tumor-specific methylation in three promoter regions. Amplicons of P1 and P2 are cut with BstUI (left and middle), and TaqI digestion is applied to the PCR products of the P3 region (right). N: normal; T: tumor; M: marker.
Acun et al. BMC Cancer 2011 11:223 doi:10.1186/1471-2407-11-223