Enhanced stabilisation of microtubules. PC3 cells were treated with docetaxel (D) or reovirus (R), alone or in combination (R+D) for 48 or 72 h (a) and levels of α-tubulin and acetylated α-tubulin were assessed by Western blot. Both treatments alone led to an increase in acetylated α-tubulin compared to untreated cells (C). When applied in combination, there was an even greater increase. Levels of acetylated α-tubulin increased for each treatment in a time dependent manner. Protein samples were additionally collected at 48 h post treatment from PC3 cells treated with paclitaxel (P), doxorubicin (Dox), cisplatin (Cis) and vincristine (V) and assessed in the same way (b). Treatment with paclitaxel and vincristine led to an increase in acetylated α-tubulin above untreated cells, whereas treatment with doxorubicin and cisplatin did not. Only treatment with paclitaxel in combination with reovirus produced levels of acetylated α-tubulin greater than that of reovirus infection alone.
Heinemann et al. BMC Cancer 2011 11:221 doi:10.1186/1471-2407-11-221