Transgene expression under survivin and cytomegalovirus promoters in UM-UC-3 bladder carcinoma and UROtsa cell lines. 50% confluent UM-UC-3 and UROtsa cells (2 × 104 cells/well) were infected in a 1 ml volume with 6 × 105 IU of (A) Ad.CMV.GFP or (B) Ad.Surv.GFP, and analyzed for GFP expression 72 h after infection by flow cytometry. Ad.Surv.GFP expression was corrected for infectivity relative to UM-UC-3 cells, determined for each experiment by Ad.CMV.GFP results. Data shown are the mean ± SD from three independent experiments. P-value was calculated by the student t test.
Murali et al. BMC Cancer 2011 11:168 doi:10.1186/1471-2407-11-168