Downregulation of STAT3 via FLLL32 treatment decreased expression of VEGF, MMP2, and survivin. A) Canine (OSA8) or human (SJSA) OSA cell lines were treated with DMSO, 10 μM curcumin, or 10 μM FLLL32 for 12 or 24 hours. RNA was collected and RT-PCR was performed for VEGF, MMP2, and GAPDH. Experiments were repeated two times. B) Canine (OSA8, OSA16, and D17) or human (SJSA and U2OS) OSA cell lines were treated with DMSO, curcumin, or FLLL32 for 24 hours prior to collection. Protein lysates were generated and separated by SDS-PAGE and western blotting for survivin, VEGF, and β-actin was performed. Experiments were repeated two times.
Fossey et al. BMC Cancer 2011 11:112 doi:10.1186/1471-2407-11-112