Table 2

Primers' sequences

mRNA

forward primer

reverse primer


HAX1 variant I

5'-GACCTCGGAGCCACAGAGAT-3'

5'-GGTGCTGAGGACTATGGAAC-3'

GenBank: NM_006118


HAX1 variant II

5'-GGACCTCGGAGCTTCAG-3'

5'-TGACTCAGGACCTGGAAGTT-3' (semi-qPCR)

GenBank: NM_001018837

5'-CCATATCGCTGAAGATGCTA-3' (qPCR)


HAX1 variant III

5'-GACCTCGGAGGTGAGA-3'

5'-CCATATCGCTGAAGATGCTA-3'

GenBank: EU190983


HAX1 variant IV

5'-AGGAATTTGGCTTCGGCTTC-3' [26]

5'-TGCAGAAAGGTGGCAGGTGTT-3' [26]

GenBank: EU190982 and HAX1 variant V VEGA:OTTHUMT00000087654


CTSD

5'-GCTGTGAGGCCATTGTGGAC-3'

5'-GCGACACCTTGAGCGTGTAG-3'

GenBank: NM_001909


GAPDH

5'-GGTCGGAGTCAACGGATTTG-3' [27]

5'-ATGAGCCCCAGCCTTCTCCAT-3' [27]

GenBank: NM_002046


ACTB

5'-AGCCTCGCCTTTGCCGA-3' [28]

5'-GCGCGGCGATATCATCATC-3' [28]

GenBank: NM_001101


Sequences of the primers used in reverse transcription semi-quantitative and quantitative PCR. GeneBank accession numbers are indicated for each transcript except for variant V where transcript ID from Vertebrate Genome Annotation (VEGA) database is shown.

Trebinska et al. BMC Cancer 2010 10:76   doi:10.1186/1471-2407-10-76

Open Data