FACS analysis of side population (SP) in the CNE-2 cell line labeled with Hoechst 33342. (A and B) SP cells exist in CNE-2 single clone cells at a various percentage; (C) One clone contains little SP cell; (D) SP cells are increased by cultured with macrophage-like cells. The bottom panel shows the reduction of SP after treatment with 50 μM of verapamil. The data are representative of experiments over three times with similar results.
Cao et al. BMC Cancer 2010 10:68 doi:10.1186/1471-2407-10-68