RT-qPCR analysis of marker gene expression in peripheral blood. Gene expression was analyzed in blood samples taken from patients (triangles) with recurrent breast cancer (A), and in blood samples taken at first diagnosis from breast (B), endometrial (C), cervical (D) and ovarian (E) cancer patients. Blood from healthy females (circles) served as a control group. Mononuclear cells were enriched with the Oncoquick density gradient. RT-qPCR was performed following a RNA pre-amplification step. Average Ct values obtained from duplicates were normalized to GAPDH gene expression. Cut-off threshold values calculated from the mean average normalized gene expression in healthy female blood as indicated by horizontal lines for the respective gene markers (DKFZp762E1312 1.39, SLC6A8 2.92, PPIC 3.61, EMP2 6.84, MAL2 14.61, CCNE2 16.83).
Obermayr et al. BMC Cancer 2010 10:666 doi:10.1186/1471-2407-10-666