PDGF-B-induced tumors can be propagated in culture also in the absence of NG2 expression .(A-H) Immunofluorescence stainings of NG2-KO (A-D) and wild type (E-H) glioma cultures with anti-GFP antibody in green, DAPI for nuclear staining in blue and the antibody for the indicated antigen in red. (I) Histogram showing the percentage of cells expressing the indicated markers in NG2-KO (red bars) and wild type (blue bars) glioma cell cultures. (L-M) Bright field microphotographs of NG2-KO (L) and wild type (M) glioma cells showing the ability to form foci in vitro. Scale bars: 50 μm (A-D); 100 μm (L-M).
Terrile et al. BMC Cancer 2010 10:550 doi:10.1186/1471-2407-10-550