Subcellular localization of claudin-1, E-cadherin, and β-catenin in MCF-7 cells after tamoxifen or claudin-1 siRNA treatment. (A) MCF-7 cells were treated with or without 40 μM of tamoxifen for 20 h, fixed, incubated with anti-claudin-1, E-cadherin or β-catenin antibody, and visualized using Alexa488-conjugated secondary antibody (Green). The cells were also counterstained with DAPI (Blue) in order to localize the nucleus. A merged image that is representative of at least two independent experiments with similar results is shown. White dot box for high magnification were also shown. (B) MCF-7 cells were transfected with siRNA against claudin-1. After 48 h incubation, the cells were fixed, incubated with anti-E-cadherin or β-catenin antibody, and visualized using Alexa488. One representative merged image is shown.
Akasaka et al. BMC Cancer 2010 10:548 doi:10.1186/1471-2407-10-548