Effect of TGZ on the expression and activity of MMP-2 within the tumor. (A) Immunohistochemistry for MMP-2 was performed using the primary tumor sections. The values given in photographs show the MMP-2-labeling index and are the means ± SE for five determinations in the control group and ten determinations in the TGZ group. *p < 0.01 (compared with the control group). (B) The activity of MMP-2 in the supernatant (10 μg of protein/lane) of tumor homogenate was assayed using gelatin zymography. The upper panel shows a representative gelatine zymogram. Arrowhead in the upper panel shows the proform of MMP-2. Values given in the lower panel are the means ± SE for ten tumor specimens in the control group and fourteen tumor specimens in the TGZ group. *p < 0.05 (compared with the control group).
Aizawa et al. BMC Cancer 2010 10:51 doi:10.1186/1471-2407-10-51