Figure 4.

Effect of TGZ on the levels of Akt and p-Akt. (A) Subconfluent LM8 cells were treated for 3 days with TGZ at the indicated concentrations. The proteins (15 μg of protein/lane) in the supernatant of cell homogenates were separated by SDS-PAGE and the expression of Akt and p-Akt was analyzed by Western blot. (B) Subconfluent LM8 cells were treated for 3 days with or without 50 μM TGZ. The expression of Akt and p-Akt was analyzed by Western blot, and relative densitometric units were determined using the analysis software. Values given are the means ± SE for five plates. *p < 0.01 (compared with the untreated cultures).

Aizawa et al. BMC Cancer 2010 10:51   doi:10.1186/1471-2407-10-51
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