Effect of TGZ on cell invasion (A), cell motility (B), and MMP-2 secretion (C). (A and B) Subconfluent LM8 cells were treated for 3 days with or without 50 μM TGZ and were harvested by trypsinization. Cell invasion and motility assays were performed using either filters coated with matrigel basement membrane matrix (A) or uncoated filters (B). Values given are the means ± SE for six filters. *p < 0.01 (compared with the untreated cultures). (C) Subconfluent LM8 cells were treated for 3 days with or without 50 μM TGZ and the activity of MMP-2 secreted into the medium during the last 24 h of the 3-day treatment was assayed using gelatin zymography. The upper panel shows a gelatine zymogram. Arrowhead in the upper panel shows the proform of MMP-2. Values given in the lower panel are the means ± SE for four plates. *p < 0.01 (compared with the untreated cultures).
Aizawa et al. BMC Cancer 2010 10:51 doi:10.1186/1471-2407-10-51