Western blot analysis of Bcl-2 family members (Bak1, Bcl-2, and Bax) and p-p53. The expression of Bak1, Bax, Bcl-2, and β-actin proteins in SNU-719 cells was investigated. Sequential treatment with 5-FU followed by LY2940002 significantly reduced the expression of Bcl-2 protein but slightly increased the expression of Bak1. However, there was no change in the expression of Bax protein.
Shin et al. BMC Cancer 2010 10:425 doi:10.1186/1471-2407-10-425