Open Access Highly Accessed Research article

SLUG/SNAI2 and Tumor Necrosis Factor Generate Breast Cells With CD44+/CD24- Phenotype

Poornima Bhat-Nakshatri1, Hitesh Appaiah1, Christopher Ballas2, Patricia Pick-Franke4, Robert Goulet1, Sunil Badve3, Edward F Srour2 and Harikrishna Nakshatri14*

Author Affiliations

1 Department of Surgery, Indiana University School of Medicine, West Walnut Street, Indianapolis, IN 46202, USA

2 Department of Medicine, Indiana University School of Medicine, West Walnut Street, Indianapolis, IN 46202, USA

3 Department of Pathology, Indiana University School of Medicine, Barnhill Drive, Indianapolis, IN 46202, USA

4 Department of Biochemistry and Molecular Biology, Barnhill Drive, Indiana University School of Medicine, Indianapolis, IN 46202, USA

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BMC Cancer 2010, 10:411  doi:10.1186/1471-2407-10-411

Published: 6 August 2010

Additional files

Additional file 1:

Table 1: List of primers used for RT-PCR. This file contains sequence of primers used for polymerase chain reactions.

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Additional file 2:

Table 2: List of genes differentially expressed in CD44+/CD24- and CD44-/CD24+ subpopulation of MCF-10A cells. This files contains list of genes that showed statistically significant difference in expression (p < 0.001, ≥ 2-fold change) between CD44+/CD24- and CD44-/CD24+ cells. Fold change in negative value corresponds to genes expressed at higher levels in CD44+/CD24- cells and in positive values corresponds to genes expressed at higher levels in CD44-/CD24+ cells.

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Additional file 3:

Figure S1: Ingenuity pathway analysis of genes expressed at higher levels in CD44+/CD24- cells. The majority of genes in this pathway are linked to signaling by follicular stimulating hormone (FSH). Shaded symbols indicate genes that are expressed at higher levels.

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Additional file 4:

Figure S2: Ingenuity pathway analysis of genes expressed at higher levels in CD44+/CD24- cells. The second signaling network links highly expressed genes in CD44+/CD24- cells to ERK and human chorionic gonadotropin (hCG) signaling.

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Additional file 5:

Figure S3: Ingenuity pathway analysis of genes expressed at higher levels in CD44+/CD24- cells. The third signaling network links highly expressed genes in CD44+/CD24- cells to p38, JNK and interferon alpha.

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Additional file 6:

Figure S4: Ingenuity pathway analysis of genes expressed at higher levels in CD44+/CD24- cells. The fourth signaling pathway links highly expressed genes in CD44+/CD24- cells to NF-κB.

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Additional file 7:

Figure S5: Ingenuity pathway analysis of genes expressed at higher levels in CD44+/CD24- cells. The fifth signaling pathway links highly expressed genes in CD44+/CD24- cells to BRCA1 and estrogen receptor (ESR1).

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Additional file 8:

Figure S6: Ingenuity pathway analysis of genes expressed at lower levels in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in this network are linked to hCG and FSH

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Additional file 9:

Figure S7: Ingenuity pathway analysis of genes expressed at lower levels in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in this network are linked to NF-κB and IL-12

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Additional file 10:

Figure S8: Ingenuity pathway analysis of genes expressed at lower levels in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in this network are linked to PI3 kinase/AKT

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Additional file 11:

Figure S9: Ingenuity pathway analysis of genes expressed at lower levels in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in this network are linked to multiple signaling molecules including growth hormone (GH1) and GRB2.

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Additional file 12:

Figure S10: Ingenuity pathway analysis of genes expressed at lower levels in CD44+/CD24- cells compared to CD44-/CD24+ cells. Genes in this network are linked to ERBB2 and TGFβ1.

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Additional file 13:

Figure S11: The effect of Gli-2 and SATB1 overexpression on CD44 and CD24 cell surface expression. This figure shows the inability of Gli-2 and SATB-1 to alter cell surface CD44 and CD24 expression profile, mammosphere formation, and EMT-associated gene expression.

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Additional file 14:

Legend for figures and table in additional files. This file provides detailed legend for all figures and tables, particularly Figure S11.

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