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Open Access Highly Accessed Research article

miR-183 inhibits TGF-β1-induced apoptosis by downregulation of PDCD4 expression in human hepatocellular carcinoma cells

Jipeng Li12, Hanjiang Fu1*, Chengwang Xu1, Yi Tie1, Ruiyun Xing1, Jie Zhu1, Yide Qin2, Zhixian Sun1 and Xiaofei Zheng1*

Author Affiliations

1 Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, People's Republic of China

2 Department of Biochemistry and Molecular Biology, Anhui Medical University, 81 Meishan Road, Hefei 230032, People's Republic of China

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BMC Cancer 2010, 10:354  doi:10.1186/1471-2407-10-354

Published: 6 July 2010

Abstract

Background

In recent years, some miRNAs have been reported to be connected closely with the development of human hepatocellular carcinoma. In our previous studies, a set of miRNAs were revealed to be dysregulated in HCC tissues. However, the functions of these miRNAs in HCC remain largely undefined.

Methods

The expression profiles of miR-183 were compared between HCC tissues and adjacent normal liver tissues using qRT-PCR method. This method was used to screen the potential target genes of miR-183. A luciferase reporter assay was conducted to confirm target association. Finally, the functional effect of miR-183 in hepatoma cells was examined.

Results

Among the 25 HCC samples analyzed, microRNA-183 was significantly up-regulated (twofold to 367-fold) in 17 samples compared with the matching nontumoral liver tissues. Programmed cell death 4 (PDCD4) was identified as the target gene of miR-183. Moreover, PDCD4 is a proapoptotic molecule involved in TGF-β1-induced apoptosis in human HCC cells, we found that miR-183 transfectants were resistant to apoptosis induced by TGF-β1.

Conclusions

We conclude that miR-183 can inhibit apoptosis in human HCC cells by repressing the PDCD4 expression, and miR-183 may play an important role in HCC development.