Table 2 |
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|
Primer sequences for PCR |
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|
Gene |
Purpose |
Sense primer |
Antisense primer |
|
|
|||
|
BLU |
Q-PCR |
CACGAGGCCTCCATCATCA |
ACTCACACACCTCCTTGTGGAA |
|
CASP8 |
Q-PCR |
TGCAGAGGGAACCTGGTACAT |
TCGAGGACATCGCTCTCTCA |
|
GAPDH |
Q-PCR |
CCATGGGGAAGGTGAAGGTCGGACTC |
GGTGGTGCAGGCATTGCTGATG |
|
HIC-1 |
Q-PCR |
TGCTGCAGCTCAACAACCA |
GGCGTTCTGCACCACGAT |
|
HIN-1 |
Q-PCR |
CACCCTCAACCCGCTGAA |
ACACTTCTGGGAGCCCTCTATG |
|
SPARC |
Q-PCR |
TCTTCCCTGTACACTGGCAGTTC |
AGCTCGGTGTGGGAGAGGTA |
|
THBS1 |
Q-PCR |
TGGAACTATGGGCTTGAGAAAAC |
CACTGATGCAAGCACAGAAAAGA |
|
TIG-1 |
Q-PCR |
CCTGGCAAACCTCTTAAAGTGAA |
GGAGGCTTCTTCTGGTGTCTGT |
|
Actin |
QMSP |
TGGTGATGGAGGAGGTTTAGTAAGT |
AACCAATAAAACCTACTCCTCCCTTAA |
|
BLU |
QMSP |
GTAGTTATGGAAACGGGTTAGTC |
CGCTAAAAATCCAAATACTATAACG |
|
CASP8 |
QMSP |
AGGTAGGAGAATCGTTTGAATTC |
AACGAAATTTCGCTCTTATTACC |
|
HIC-1 |
QMSP |
GTTAGGCGGTTAGGGCGTC |
CCGAACGCCTCCATCGTAT |
|
HIN-1 |
QMSP |
AGGGACGATTAGGTTTTATTTTC |
CTAATTTCGAAAACAAAAAAACG |
|
MYOD1 |
QMSP |
CCAACTCCAAATCCCCTCTCTAT |
TGATTAATTTAGATTGGGTTTAGAGAAGGA |
|
SPARC |
QMSP |
GTTGTTATATTCGGGGACGAC |
TAACCCGTTTCCATAACTACGA |
|
THBS1 |
QMSP |
GAAAGGGTTCGAAGGTAGC |
CCGAACGCCTATCCTAAA |
|
TIG-1 |
QMSP |
GTTTAGTGTTAGGATGCGGTATC |
AAAACGCGATACGAAATAACA |
|
THBS1 |
ChIP-1 |
ATGACAACTTGGCAAAAAAGAGAA |
GGCCGAAGTGATGGACCTT |
|
THBS1 |
ChIP-2 |
AATGTGGGTGAATTCCTGTTAAGG |
GGGTGGAAAGGAAAGGTCATAGA |
|
THBS1 |
ChIP-3 |
TGGGTGCCGATTCCAGAA |
TCCCGCAAATCATAGGTAATGA |
|
THBS1 |
ChIP-4 |
CCACGCAAGAAAAGCGAAA |
TGCTGCAAACAGCCAAGTG |
|
THBS1 |
ChIP-5 |
GGGCAGGTACTTTAACGAATGG |
CTGGGCCCGTTTTGTAAAAA |
|
THBS1 |
ChIP-6 |
GGCGGGCACCGACTTT |
GCGCAACTTTCCAGCTAGAAA |
|
|
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|
Yang et al. BMC Cancer 2010 10:286 doi:10.1186/1471-2407-10-286 |
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