Email updates

Keep up to date with the latest news and content from BMC Cancer and BioMed Central.

Open Access Research article

A genomic and transcriptomic approach for a differential diagnosis between primary and secondary ovarian carcinomas in patients with a previous history of breast cancer

Jean-Philippe Meyniel1*, Paul H Cottu12, Charles Decraene234, Marc-Henri Stern35, Jérôme Couturier6, Ingrid Lebigot6, André Nicolas6, Nina Weber6, Virginie Fourchotte7, Séverine Alran7, Audrey Rapinat1, David Gentien1, Sergio Roman-Roman1, Laurent Mignot2 and Xavier Sastre-Garau6

Author Affiliations

1 Institut Curie, Department of Translational Research, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

2 Institut Curie, Department of Medical Oncology, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

3 Institut Curie, Research Unit, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

4 CNRS, UMR144, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

5 INSERM, U830, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

6 Institut Curie, Department of Pathology, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

7 Institut Curie, Department of Surgery, 26 rue d'Ulm, 75248 Paris, Cedex 05, France

For all author emails, please log on.

BMC Cancer 2010, 10:222  doi:10.1186/1471-2407-10-222

Published: 21 May 2010

Abstract

Background

The distinction between primary and secondary ovarian tumors may be challenging for pathologists. The purpose of the present work was to develop genomic and transcriptomic tools to further refine the pathological diagnosis of ovarian tumors after a previous history of breast cancer.

Methods

Sixteen paired breast-ovary tumors from patients with a former diagnosis of breast cancer were collected. The genomic profiles of paired tumors were analyzed using the Affymetrix GeneChip® Mapping 50 K Xba Array or Genome-Wide Human SNP Array 6.0 (for one pair), and the data were normalized with ITALICS (ITerative and Alternative normaLIzation and Copy number calling for affymetrix Snp arrays) algorithm or Partek Genomic Suite, respectively. The transcriptome of paired samples was analyzed using Affymetrix GeneChip® Human Genome U133 Plus 2.0 Arrays, and the data were normalized with gc-Robust Multi-array Average (gcRMA) algorithm. A hierarchical clustering of these samples was performed, combined with a dataset of well-identified primary and secondary ovarian tumors.

Results

In 12 of the 16 paired tumors analyzed, the comparison of genomic profiles confirmed the pathological diagnosis of primary ovarian tumor (n = 5) or metastasis of breast cancer (n = 7). Among four cases with uncertain pathological diagnosis, genomic profiles were clearly distinct between the ovarian and breast tumors in two pairs, thus indicating primary ovarian carcinomas, and showed common patterns in the two others, indicating metastases from breast cancer. In all pairs, the result of the transcriptomic analysis was concordant with that of the genomic analysis.

Conclusions

In patients with ovarian carcinoma and a previous history of breast cancer, SNP array analysis can be used to distinguish primary and secondary ovarian tumors. Transcriptomic analysis may be used when primary breast tissue specimen is not available.