Research article
Expression analysis of genes associated with human osteosarcoma tumors shows correlation of RUNX2 overexpression with poor response to chemotherapy
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* Corresponding author: Jeremy Squire squire@queensu.ca
1 Department of Pediatric Laboratory Medicine, Pathology Division, Hospital for Sick Children, Toronto, M5G 1X8 Canada
2 Genetics and Genome Biology Program, Hospital for Sick Children, Toronto, ON, Canada
3 Division of Applied Molecular Oncology, Ontario Cancer Institute, the University Health Network, Toronto, M5G 2M9 Canada
4 Department of Pathology and Molecular Medicine, Richardson Labs, Queen's University, Kingston, K7L 3N6 Canada
BMC Cancer 2010, 10:202 doi:10.1186/1471-2407-10-202
Published: 13 May 2010Additional files
Additional file 1:
Primer sequences. Primer sequences for Real-Time PCR
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Additional file 2:
Gene expression levels in osteosarcoma tumors and normal osteoblasts. Values of gene expression are represented as delta-delta Ct (log10).
Format: XLS Size: 21KB Download file
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Additional file 3:
Controls for qRT-PCR analyses. Respectively, A2M and SLC14A were the highest- and lowest- expressed genes in a previous microarray study performed by our group [19], and they were used to validate our conditions for the qRT-PCR experiments of the current study.
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Additional file 4:
Detailed results of the statistical analyses of gene expression. Tables represent values calculated using Mann-Whitney and 1-way ANOVA statistical analyses respectively
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