Table 2

Details of candidate reference genes and their amplification efficiencies

Name

Length (nt)

RNA species

Accession number

Function

E (%)


let-7a

22

miRNA

MI0000060*

Negatively regulates RAS oncogene [37]

100.0


miR-16

22

miRNA

MI0000070*

Negatively regulates B-cell lymphoma mRNA in chronic lymphocytic leukaemia [38]

100.0


miR-26a

22

miRNA

MI0000083*

Involved in myogenesis and osteogenic differentiation [39,40]

99.8


miR-345

22

miRNA

MI0000825*

Overexpressed in malignant mesothelioma [41]

100.8


miR-425

22

miRNA

MI0001448*

No functionally verified targets

101.2


miR-454

22

miRNA

MI0003820*

No functionally verified targets

101.8


RNU48

63

snoRNA

NR_002745**

Guides the 2'O-ribose methylation of 28S rRNA [42]

100.0


Z30

97

snoRNA

AJ007733**

Guides the methylation of the Am47 residue in U6 snoRNA [43]

99.4


*mirBase database accession number **Entrez gene ID

rRNA, ribosomal RNA; snoRNA, small nucleolar RNA; E, amplification efficiency.

Chang et al. BMC Cancer 2010 10:173   doi:10.1186/1471-2407-10-173

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