Table 5

Quantitative real-time PCR analysis of zinc finger transcription factor expression in tumor tissue relative to non-neoplastic tissuea.

Clinical stage


T3

T2


Gene

Up

in tumorb,c

No change/Downb,d

Up

in tumorb,c

No change/Downb,d


WT1

7

3

3

7

EGR-1

0

10

2

8

GATA-2

4

6

2

8


a: Frozen tumor and non-neoplastic tissue obtained from 20 patients with prostate cancer (all Gleason Score 7, but clinical stage ranging from T2 to T3b). Gene expression levels determined by the ddCt method after normalization using 18s rRNA primers.

b. Number of samples with elevated expression (Up in tumor) and those with no change or reduced expression (No change/Down) in tumor relative to non-neoplastic tissue is shown for each gene and clinical stage.

c. Up in tumor: Tumor samples with elevated expression, relative to non-neoplastic tissue. Fold-change in expression values ≥ 2.0;

d. No change/Down: Tumor samples with no change or reduced expression, relative to non-neoplastic tissue. Fold-change in expression values ≥ 2.0 or no difference from non-neoplastic tissue.

Gregg et al. BMC Cancer 2010 10:165   doi:10.1186/1471-2407-10-165

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