Expression levels of selected IFNα/β pathway genes as a function of PSCA expression. Expression levels for IFI27, IFI44L and IFIT1 genes were measured in individual SW780-shPSCA tumors (n = 6-8) (A) or in SW780-shPSCA in cell culture conditions (B) with or without dox treatment. Gene expression was measured by qPCR using the ΔΔ ct method. The average gene values in dox untreated mice served as reference and GAPDH gene as normalizer. In black average values in SW780-shPSCA tumors and in white SW780-shCont.
Marra et al. BMC Cancer 2010 10:129 doi:10.1186/1471-2407-10-129