Figure 2.

Northern blot analysis of nmt55/p54^nrb mRNA expression in MCF-7 cells and ER+/ER- Human Breast tumors. Total RNA (20 μg) was prepared from MCF-7 cells (lane M) and twelve different human breast tumors (lanes 1–12). The RNA was subjected to hybridization with a human nmt55/p54^nrb 499 bp SacI/BglII radiolabeled probe (top panel) or a human glyceraldehyde-3-phosphate (GAPDH) 545 bp HindIII/Xbal radiolabeled probe to indicate levels of RNA loading (bottom panel). Estrogen receptor (ER) expression levels were determined by ligand binding assay and are indicated along the top of the figure.