Drug-induced phospholipidosis is determined by measuring the accumulation of a fluorescent phospholipid in cells treated with increasing drug concentrations. Fluorescence is measured and normalized to cell number. Fluorescence is increased in cells treated with compounds that are known to cause phospholipidosis (chlorpromazine, tamoxifen, amiodarone), but it is not increased in cells treated with a compound that is known not to cause phospholipidosis (acetaminophen). Conc., concentration.
Tsaioun et al. BMC Neurology 2009 9(Suppl 1):S1 doi:10.1186/1471-2377-9-S1-S1