Fluorescent immunohistochemical double-labelling of serial sections in GNE myopathy muscle biopsy specimen. A representative GNE myopathy sample is shown with five consecutive sections that were stained for hematoxylin/eosin (first row); APP (red) and MHC-I (green) (second row); αB-crystallin (red) and NCAM (green) (middle row); IL-1β (red) and β-amyloid (green) (fourth row); iNOS (red) and P-neurofilament (green) (last row). The highest number of positive staining signal is noted for iNOS, followed by αB-crystallin and NCAM. All other markers are present only in very few fibers.
Fischer et al. BMC Neurology 2013 13:24 doi:10.1186/1471-2377-13-24