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Characteristics of progressive multifocal leukoencephalopathy clarified through internet-assisted laboratory surveillance in Japan

Kazuo Nakamichi1, Hidehiro Mizusawa2, Masahito Yamada3, Shuji Kishida4, Yoshiharu Miura4, Toshio Shimokawa5, Tomohiko Takasaki1, Chang-Kweng Lim1, Ichiro Kurane1 and Masayuki Saijo1*

Author Affiliations

1 Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan

2 Department of Neurology and Neurological Science, Graduate School, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo, 113-8519, Japan

3 Department of Neurology and Neurobiology of Aging, Kanazawa University Graduate School of Medical Science, Ishikawa, Kanazawa, 920-8640, Japan

4 Division of Neurology, Tokyo Metropolitan Cancer and Infectious diseases Center Komagome Hospital, Bunkyo-ku, Tokyo, 113-8677, Japan

5 Department of Ecosocial System Engineering, Interdisciplinary Graduate School of Medicine and Engineering, University of Yamanashi, Kofu, Yamanashi, 400-8511, Japan

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BMC Neurology 2012, 12:121  doi:10.1186/1471-2377-12-121

Published: 15 October 2012

Additional files

Additional file 1:

Figure S1. Schematic diagram of the standard DNA and primer / probe sets for PCR testing. Yellow and grey lines represent the sequences of the JCV genome and pBR322 vector within the standard DNA (pJC1-4->pJCV), respectively. The numbers in the circle correspond to the nucleotide positions within the JCV genome. Three primer / probe sets detect the JCV T and VP1 genes and the boundary sequence of the JCV genome and pBR322 (green, red, and blue, respectively).

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Additional file 2:

Figure S2. Examples of real-time PCR amplifications. Three real-time PCR assays were designed to detect the JCV T (A) and VP1 (B) sequences and the contamination of samples with standard DNA (C). The reactions were performed in the absence or presence of standard DNA (2.0 x 108 to 0.8 copies per reaction). Relative fluorescence is plotted against cycle number. These PCR assays were capable of detecting at least 4 copies of JCV DNA per reaction under the same conditions. The data are representative of three independent experiments.

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