Email updates

Keep up to date with the latest news and content from BMC Neurology and BioMed Central.

Open Access Research article

Introduction of a new model for time-continuous and non-contact investigations of in-vitro thrombolysis under physiological flow conditions

Florian C Roessler1*, Marcus Ohlrich1, Jan H Marxsen2, Marc Schmieger3, Peter-Karl Weber3, Florian Stellmacher4, Peter Trillenberg1, Jürgen Eggers1 and Günter Seidel5

Author Affiliations

1 Department of Neurology, University Hospital of Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

2 Department of Internal Medicine, Haematology, University Hospital of Schleswig-Holstein, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany

3 Fraunhofer Institute for Biomedical Engineering (IBMT), Ultrasound Systems Development, Ensheimer Str. 48, 66386 St. Ingbert, Germany

4 Research Centre Borstel, Clinical and Experimental Pathology, Parkallee 3a, 23845 Borstel, Germany

5 Department of Neurology, Asklepios Klinik Nord, Langstedter Landstr. 400, 22417 Hamburg, Germany

For all author emails, please log on.

BMC Neurology 2011, 11:58  doi:10.1186/1471-2377-11-58

Published: 26 May 2011

Abstract

Background

Thrombolysis is a dynamic and time-dependent process influenced by the haemodynamic conditions. Currently there is no model that allows for time-continuous, non-contact measurements under physiological flow conditions. The aim of this work was to introduce such a model.

Methods

The model is based on a computer-controlled pump providing variable constant or pulsatile flows in a tube system filled with blood substitute. Clots can be fixed in a custom-built clot carrier within the tube system. The pressure decline at the clot carrier is measured as a novel way to measure lysis of the clot. With different experiments the hydrodynamic properties and reliability of the model were analyzed. Finally, the lysis rate of clots generated from human platelet rich plasma (PRP) was measured during a one hour combined application of diagnostic ultrasound (2 MHz, 0.179 W/cm2) and a thrombolytic agent (rt-PA) as it is commonly used for clinical sonothrombolysis treatments.

Results

All hydrodynamic parameters can be adjusted and measured with high accuracy. First experiments with sonothrombolysis demonstrated the feasibility of the model despite low lysis rates.

Conclusions

The model allows to adjust accurately all hydrodynamic parameters affecting thrombolysis under physiological flow conditions and for non-contact, time-continuous measurements. Low lysis rates of first sonothrombolysis experiments are primarily attributable to the high stability of the used PRP-clots.