Open Access Highly Accessed Research article

Intracellular calcium release modulates polycystin-2 trafficking

Ayako Miyakawa12, Cristián Ibarra1, Seth Malmersjö1, Anita Aperia3, Peter Wiklund2 and Per Uhlén1*

Author Affiliations

1 Department of Medical Biochemistry and Biophysics, Karolinska Institutet, SE-171 77, Stockholm, Sweden

2 Department of Molecular Medicine and Surgery, Karolinska University Hospital, SE-171 76, Stockholm, Sweden

3 Department of Woman and Child Health, Astrid Lindgren Children’s Hospital Q2:09, Karolinska University Hospital, SE-171 76, Stockholm, Sweden

For all author emails, please log on.

BMC Nephrology 2013, 14:34  doi:10.1186/1471-2369-14-34

Published: 11 February 2013

Additional files

Additional file 1: Figure S1:

PC2 expression pattern in proximal tubule cells using two different antibodies. (A-D) Immunocytochemistry of PC2 in rat proximal tubule cells treated with control (A,C) or 100 μM ouabain (B,D) using anti-PC2 polyclonal antibodies against amino acids 103 to 203 (YCB9) or 44 to 62 on the N-terminus. Scale bars, 20 μm.

Format: TIFF Size: 2MB Download file

Open Data

Additional file 2: Figure S2:

Immunocytochemistry negative control without primary PC2 antibody. (A-B) Immunocytochemistry staining in rat proximal tubule cells without (A) and with (B) anti-PC2 antibody present. Scale bars, 20 μm.

Format: TIFF Size: 1MB Download file

Open Data