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Open Access Research article

Antibodies to hepatitis B virus surface antigen and interleukin 12 and interleukin 18 gene polymorphisms in hemodialysis patients

Alicja E Grzegorzewska1*, Piotr M Wobszal1, Adrianna Mostowska2 and Paweł P Jagodziński2

Author Affiliations

1 Chair and Department of Nephrology, Transplantology and Internal Diseases Poznań University of Medical Sciences, 49 Przybyszewskiego Blvd, 60-355, Poznań, Poland

2 Department of Biochemistry and Molecular Biology, Poznań University of Medical Sciences, Poznań, Poland

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BMC Nephrology 2012, 13:75  doi:10.1186/1471-2369-13-75

Published: 3 August 2012

Abstract

Background

The interleukin (IL)18 rs360719 CC genotype is associated with the development of antibodies to hepatitis B virus surface antigen (anti-HBs) in hemodialysis (HD) patients. IL18 shares biological properties with IL12 in promoting the T-hepler 1 (Th1) system. We studied whether polymorphisms in the IL12A 3` untranslated region (UTR) and IL12B 3`UTR may contribute to anti-HBs development (titre ≥ 10 IU/L) in HD patients either individually or jointly with the IL18 polymorphism.

Methods

In 518 HD patients and 240 controls the IL12A rs568408 3’UTR G > A polymorphism was genotyped by high-resolution melting curve analysis. Polymerase chain reaction restriction fragment length polymorphism was used to detect the IL12B rs3212227 3’UTR A > C and IL18 -1297 T > C rs360719 polymorphisms. The associations between the IL12A, IL12B and IL18 genotypes and the risk of impaired anti-HBs development were estimated by computing the odds ratios and their 95% confidence intervals using logistic regression analysis.

Results

In the logistic regression analysis, the higher frequency of rs360719 CC individually (2.9% in 207 patients without anti-HBs development vs 8.0% in 311 patients with anti-HBs development, p = 0.009) and of rs360719 CC combined with rs568408 GG (p = 0.048), rs568408 GA (p = 0.035), rs568408 GG/AA (p = 0.034) or rs3212227 AA (p = 0.046) was associated with an increased chance for the development of anti-HBs in HD patients. Patients bearing both rs568408 AA and rs360719 TT had a 10.9-fold or 8.9-fold lower chance, respectively, to develop anti-HBs compared with those carrying any other genotype (p = 0.005) or those who had both wild-type rs568408 GG and rs360719 TT (p = 0.011). Carriers of both rs3212227 CC and rs360719 TC had a 4.6-fold lower chance for anti-HBs development than carriers of any other genotype (p = 0.042).

Conclusion

Development of anti-HBs in HD patients is associated with gene polymorphisms of interleukins involved in the Th1 system.

Keywords:
Antibodies to surface antigen of hepatitis B virus; Gene polymorphisms; Hemodialysis; Interleukin 12; Interleukin 18