Open Access Research article

Risk factors affecting seroconversion after influenza A/H1N1 vaccination in hemodialysis patients

Sung Jin Moon1, Sang Hun Lee2, Young-Ho Byun3, Gi Young Yun2, Seung Kyu Kim2, Baik-Lin Seong3, Ah Reum Kim4, Eun sun Park4, Hyung-Jong Kim5, Jung Eun Lee6, Sung Kyu Ha2, Jae Myun Lee4* and Hyeong-Cheon Park2*

Author affiliations

1 Myongji Hospital, College of Medicine, Kwandong University, Goyang, Korea

2 Department of Internal Medicine, Gangnam Severance Hospital, Institute of Vascular and Metabolism Research, College of Medicine, Yonsei University, 146-92 Dogok-Dong, Gangnam-Gu, Seoul 135-720, Korea

3 Department of Biotechnology, College of Life Science & Biotechnology, Yonsei University, Seoul, Korea

4 Department of Microbiology and Brain Korea 21 Project for Medical Sciences, Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, 134, Shinchon-dong, Seodaemoon-gu 120-752 Seoul, Korea

5 BundangCHA Medical Center, CHA University, Sungnam, Korea

6 Yongin Severance Hospital, College of Medicine, Yonsei University, Yongin, Korea

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Citation and License

BMC Nephrology 2012, 13:165  doi:10.1186/1471-2369-13-165

Published: 3 December 2012



Hemodialysis (HD) patients have multiple causes of immune dysfunction and poor immune response to influenza vaccination. We investigated the antibody response rate to a pandemic H1N1/2009 influenza vaccination and clinical parameters influencing the induction of antibody responses in HD patients.


A total of 114 HD patients were vaccinated with a monovalent adjuvanted H1N1 inactivated influenza vaccine. Titers of neutralizing antibodies were evaluated by hemagglutination inhibition (HI) assay at pre- and 4 weeks after vaccination. Seroconversion was defined as either a pre-vaccination HI titer < 1:10 and a post vaccination HI titer > 1:40 or a pre-vaccination HI titer ≥ 1:10 and a minimum four-fold rise in post-vaccination HI antibody titer. Seventeen out of 114 HD patients (14.9%) tested positive for antibodies against influenza A/H1N1/2009 before vaccination. The remaining 97 baseline sero-negative patients were included in the analysis.


Only 30 (30.9%) HD patients had seroconversion 4 weeks after vaccination. The elderly patients, those over 65 years of age, showed significantly lower seroconversion rate compared to younger HD patients (20.5% vs. 39.6%, p = 0.042). Furthermore, patients with hemoglobin values less than 10 g/dL had a significantly lower seroconversion rate compared to those with higher hemoglobin values (20.0 vs. 38.6%, p = 0.049). By multivariate logistic regression analysis, only age ≥65 years (OR = 0.336, 95% confidence interval (CI) 0.116-0.971, p = 0.044) and hemoglobin levels <10 g/dL (OR = 0.315, 95% CI 0.106-0.932, p = 0.037) were independently associated with seroconversion after vaccination.


Our data show that HD patients, especially who are elderly with low hemoglobin levels, are at increased risk for lower seroconversion rate after influenza A/H1N1 vaccination. Further studies are needed to improve the efficacy of vaccination in these high risk patients.

Hemodialysis; Pandemic H1N1/2009 influenza; Vaccine; Seroconversion