Research article

Dinucleotide repeat polymorphism in Fms-like tyrosine kinase-1 (Flt-1) gene is not associated with preeclampsia

Shin-Young Kim¹ , Ji-Hyae Lim¹ , Jae-Hyug Yang² , Moon-Young Kim² , Jung-Yeol Han² , Hyun-Kyong Ahn² , Jun-Seek Choi² , So-Yeon Park¹ , Mi-Jin Kim¹ and Hyun-Mee Ryu^1,2

¹Laboratory of Medical Genetics, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, Korea

²Department of Obstetrics and Gynecology, Cheil General Hospital and Women's Healthcare Center, Kwandong University College of Medicine, Seoul, Korea

author email corresponding author email

BMC Medical Genetics 2008, 9:68doi:10.1186/1471-2350-9-68

Published:	17 July 2008

Abstract

Background

Preeclampsia is a major cause of maternal and perinatal mortality and morbidity. The etiology of preeclampsia remains unclear. Recently, it was shown that misregulation of fms-like tyrosine kinase-1 (Flt-1) in the peripheral blood mononuclear cells of pregnant women results in over-expression of the soluble splice variant of Flt-1, sFlt-1, producing an additional (extra-placental) source of sFlt-1 that can contribute to the etiology of preeclampsia. The aim of this study was to investigate the relationship between preeclampsia and a dinucleotide (threonine-glycine; TG)_n repeat polymorphism in the 3' non-coding region of the Flt-1 gene.

Methods

The number of the d(TG)_n repeats was analyzed in 170 patients with preeclampsia and in 202 normotensive pregnancies. The region containing the dinucleotide repeat polymorphism of the Flt-1 gene was amplified by polymerase chain reaction (PCR) from the DNA samples and was analyzed by direct PCR sequencing.

Results

We found 10 alleles of the dinucleotide repeat polymorphism and designated these as allele*12 (A1) through allele*23 (A12) according to the number of the TG repeats, from 12 to 23. The frequency of the 14-repeat allele (A3) was most abundant (63.82% in preeclampsia and 69.06% in controls), followed by the 21-repeat allele (A10; 28.53% in preeclampsia and 23.76% in controls). There was no significant difference in the allele frequency between patients with preeclampsia and normal controls. The most common genotype in preeclamptic and normotensive pregnancies was heterozygous (TG)₁₄/(TG)₂₁ (41.76%) and homozygous (TG)₁₄/(TG)₁₄ (45.05%), respectively. However, the genotype frequencies were not significantly different between preeclamptic patients and controls.

Conclusion

This is the first study to characterize the dinucleotide repeat polymorphism of the Flt-1 gene in patients with preeclampsia. We found no differences in the allele or genotype frequencies between patients with preeclampsia and normal pregnancies. Although limited by a relatively small sample size, our study suggests that the d(TG)_n repeat polymorphism of the Flt-1 gene is not associated with the development of preeclampsia in Korean pregnant women.