Sequence flurograms (using reverse primer) show partial methylation of cytosine 8 and 20 in human brain specific regions. A. (cerebellar vermis) shows prominent methylation of cytosine 8 and 20 (both sites showing C and a trace of T), B. (caudate; head) shows partial methylation (N call by sequencer) of cytosines 8 and 20.
Murphy et al. BMC Medical Genetics 2008 9:115 doi:10.1186/1471-2350-9-115